Naphthalene exerts non-target effects ontheabundanceof active fungi by stimulating basidiomycete abundance Naphthalene exerts non-target effects ontheabundanceof active fungi by stimulating basidiomycete abundance

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Vol17 No.8: 2001-2010

Title】Naphthalene exerts non-target effects ontheabundanceof active fungi by stimulating basidiomycete abundance

Author】LAN Li-ying1,2#; ZHANG Li1,2#; SHEN Ya1,2; ZHANG Jian1,2;. YANG Wan-qin3; XU Zhen-feng1,2; LIUYang1,2; HE Shu-qin1,2; ZHOU Wei4; LI Han1,2; WANG Li-xia1,2; LIU Si-ning1,2; YOU Cheng-ming1,2; TAN Bo1,2*

Addresses】1 Institute of Ecology & Forestry, Sichuan Agricultural University, Chengdu 611130, China; 2 Forestry Ecological Engineering in Upper Reaches of Yangtze River Key Laboratory of Sichuan Province, Chengdu 611130, China; 3 School of Life Science, Taizhou University, Taizhou 318000, China; 4 College of Resources, Sichuan Agricultural University, Chengdu 611130, China

Corresponding author】TAN Bo

Citation】Lan LY, Zhang L, Shen Y, et al. (2020) Naphthalene exerts non-target effects on the abundance of active fungi by stimulating basidiomycete abundance. Journal of Mountain Science 17(8). https://doi.org/10.1007/s11629-020-5985-7

DOI】https://doi.org/10.1007/s11629-020-5985-7

Abstract】As an arthropod biocide, naphthalene has been used in studies of the ecological functions of soil fauna for decades. However, its potential non-target effects on soil microorganisms may affect soil mineralization and litter decomposition processes. Therefore, we conducted an experiment with naphthalene adding to soil surface at a rate of 100 g·m-2 per month to examine the potential non-target effects of this treatment on soil fungal phospholipid fatty acids (PLFAs), 18S rDNA gene copy numbers and community diversity in a subalpine forest of western Sichuan, China. The results showed that naphthalene addition significantly increased fungal PLFAs but did not significantly alter fungal gene copy numbers. A total of 16 phyla, 62 genera and 147 Operational taxonomic units (OTUs) were identified through Illumina MiSeq sequencing analysis. Basidiomycota and Ascomycota were the most abundant phyla in both the control and naphthalene addition plots. Naphthalene addition did not affect the diversity or structure of the soil fungal community, but the increase in some genera of Basidiomycota might contribute to the increase in fungal PLFAs in the naphthalene addition plots. These results suggest that naphthalene exerts non-target effects on the active fungal abundance by stimulating the abundance of specific taxa in subalpine forest soils. The non-target effects of naphthalene on the fungal community should be taken into consideration when it is used to exclude soil fauna.

Keywords】Naphthalene; Illumina sequencing; Phospholipid fatty acids; Soil fungi; Soil fauna